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Stage 2


Research focused on photosensitizers’ toxicity / efficacy profile continued in this stage with „in vitro” studies on cytotoxicity and incorporation of porphyrinic compounds P.1.2 and P.4.2. on normal human cell lines of keratinocytes (HaCaT) and dermal fibroblasts (HS27).

In respect with potential targets for photodynamic therapy new compounds’ design (cytotoxicity, antioxidant effects on normal cells surrounding the tumors, anti-carcinogenic / anti-proliferative and pro-apoptotic action), it have been developed intermediate procedures concerning preclinical investigation of nano-systems and photosensitizers, as a basis for future experimental investigations.


As study results, valuable data concerning antioxidant efficacy of chlorophyll and phyto-estrogenic vegetal extracts were achived. It have been applied experimental models that quantify the antiradicalic properties (DPPH technique), and evaluate on fibroblasts and keratinocyte cell culture antioxidant enzymes (catalase and superoxide dismutase), as well as the protective potential against UV-A and UV-B radiation.



The saponinic complex from fenugreek induced cellular protection through UV-A induced apoptosis, anti-inflammatory and anti-angiogenic effects against UV-B. The fenugreek extract with hydrolised diosgenine content has pronounced antiinflammatory effect on keratinocytes. The red-clover extract with a standardised content of isoflavones had a pronounced anti-inflammatory effect, completed with anti-apoptotic and anti-oxidative actions on UV irradiated cells. As well as, the chlorophyll complex reduces the cellular UV degradation, especially the disturbancies arrised in  cell cycle sequence.

All these findings reccomend the extracts for further investigations as co-therapy with anti-tumor nanoparticles and photosensitizers.