At this stage of the project, there have been defined optimized procedures for „in vitro”, preclinical investigations of nano-systems and photosensitizers. Preclinical studies on antitumor efficacy and toxicological profile were also finalized. As part of this research, the effect of photodynamic therapy (PDT) on human adenocarcinoma cells HT-29 viability was evaluated, testing the P2.2. porphyrinic asymmetric compound produced by UMF „Carol Davila” and incorporated in cells at INCD „Victor Babes”.
Applying specific models for antiproliferative and proapoptotic action in different conditions of B16-F10 murine melanoma cells cultivation (irradiation and non-irradiation), SC Biotehnos SA selected as promissing active principles 3 vegetal extracts with phytoestrogenic content from fenugreek and red clover. The results show that the active compounds have moderate antiproliferative and proapoptotic biological activity with minimal toxicity, thus ensuring synergism of action for photosensitizers and developed nanosystems.
Within the inter-laboratory collaboration, there is an efficient information flow on biological material testing, assay methodologies and techniques, in order to draw up a technology transfer documentation between partners as capitalization of research results.
It is opportune to associate for co-therapy biologically active compounds, in order to increase the anti-tumor efficacy of nanosystems and to limit the adverse effects of nanoparticles.